Ryu
Ken Masters
Chun-Li
Sean
Mario
Sonic
PAC-MAN
Mega Man
Meggy Spletzer
Tari
Saiko
Uzi from Murder Drones
Note: I originally wanted SMG4 in this as the leader, but his moveset will be too similar to Mario, so i replaced him with Uzi and Meggy became the leader instead.
Terry Bogard
Mai Shiranui
Kim Kaphwan
Rock Howard
Athena Asamiya
Bloom
Iris
Star Butterfly
Kyo Kusanagi
K'
Ash Crimson
Shun'ei
Goku
Monkey D. Luffy
Naruto Uzumaki
Izuku "Deku" Midoriya
Kazuya Mishima
Morrigan Aensland
Enja
Death from Castlevania
Nakoruru
Tessa
Alex Kidd
Kirito
Angel
Tizoc
King
Sombra
Dan Hibiki
Luigi
Yukino Ozawa
Belle Fontiere
Morshu
Old Man from GoodTimes' Beauty and the Beast
Bulk Bogan
Dee Bee Kaw
M. Bison
Geese Howard
Dr. Eggman
Heihachi Mishima
Iori Yagami
Melony
Icy
Bass
Blue Mary
Seth
Vanessa
Leon Kennedy
King Lion
Unlocked by landing a Super Desperation on him
Rugal Bernstein
Unlocked by landing a Climax Desperation on him
Akuma / Gouki
Unlocked by landing a Climax Desperation on him
Note: In order to unlock him, you have to land a DM to the last team member in every match (minus King Lion), doing so will have Rugal chopped in the back by Akuma's Misogi.
Baiken
Unlocked by landing a Climax Desperation on her
Note: In order to unlock her, you have to beat Akuma with a Climax without using any continues and have the last member survive. Nothing happens at first during the credits, then a slash is suddenly seen as blossom petals falls down, that's when you give the chance to fight against her. This is a homage to the original Guilty Gear where Baiken first appeared as a secret fighter.